Využití rohovkové stromální lentikuly získané při refrakčních operacích ReLEx SMILE k léčbě rohovkových vředů

Abstract

- Vyu it" rohovkov strom ln" lentikuly z"skan pYi refrak n"ch operac"ch ReLEx SMILE k l b rohovkových vYedo òvod: Rohovkov strom ln" lentikula (RSL) je st rohovkov ho stromatu, kter vznik jako odpadn" produkt pYi laserov refrak n" operaci typu ReLEx SMILE (Small Incision Lenticule Extraction), a nen" nad le vyu "v na. Se zvyauj"c"m se po tem operac" ReLEx SMILE a t"m i mno stv" potenci ln vyu itelných RSL jsou v sou asnosti zkoum ny dva hlavn" aspekty jejich vyu it". Prvn" aspekt výzkumu se týk biologických vlastnost" samotn RSL a jej" spr vn uchov n" s ohledem na dlouhodob skladov n". Druhý aspekt souvis" s mo ným klinickým vyu it"m RSL. Jako kvalitn" biomateri l m RSL zna ný potenci l k alternativn"mu pou it" v l b nkterých o n"ch onemocnn". Konkr tn l ba rohovkových vYedo je v sou asn dob obt" n vzhledem k relativn" nedostupnosti d rcovských rohovek uchov vaných technikou hypotermie, pY"padn v tk Iových m di"ch. Mo nost vyu it" hluboko zmrazených RSL by mohlo významn napomoci v tchto pY"padech. Z roveI lze pYedpokl dat mo nost dlouhodob ho uchov n" tchto RSL metodou kryoprezervace, "m by byly tyto tk n k okam it dispozici. C"l: C"lem naa" pr ce bylo zhodnotit a ovYit metodu kryoprezervace RSL, zjistit jej" vliv na vlastnosti tk n v experiment ln"ch podm"nk ch...

- Using corneal stromal lenticules obtained during ReLEx SMILE refractive surgery for the treatment of corneal ulcers Background: Corneal stromal lenticule (CSL) is a part of the corneal stroma that is generated as a waste product during the refractive surgery ReLEx SMILE (Small Incision Lenticule Extraction) and is no longer used. With the increasing number of ReLEx SMILE procedures and the number of potentially usable CSLs, two main aspects of their use are currently being investigated. The first aspect includes the biological properties of CSLs and their proper preservation with respect to long-term storage. The second aspect is related to the potential clinical use of CSLs. As a high-quality biomaterial, CSL has significant potential for alternative use in the treatment of some eye diseases. Especially the treatment of corneal ulcers is currently difficult due to the relative unavailability of donor corneas preserved by the hypothermia technique or in tissue media. The possibility of using deep-frozen CSLs could significantly help in these cases. Concurrently it can be assumed that these CSLs can be preserved for a long time by cryopreservation, which would make these tissues immediately available. Purpose: The aim of our work was to evaluate and verify the method of cryopreservation of CSL, to...