Apoptóza zárodečných buněk navozená arteficiálním kryptorchismem

Abstract

Abstrakt. Studovali jsme vliv experimentalniho unilateralniho kryptorchismu na morfologicke a histologicke ukazatele ve tkanich varlat a nadvarlat skupiny samců potkana Wistar. Doba trvani experimentu byla 7 dnů, přičemž byla do kryptorchickych varlat injikovana latka MDOCTM. Pro histologicke hodnoceni kratšich časovych intervalů jsme použili take material z jinych srovnatelnych pokusů, ale bez MDOCTM. Delka a hmotnost kryptorchickych varlat byly vyznamně sniženy v porovnani s kontrolami (22,4% a 52,6%). Degenerativni změny byly patrne již v intervalu jedne hodiny (desintegrace zarodečneho epitelu). Nezrale zarodečne buňky včetně apoptotickych jsme nalezli v lumen kanalku nadvarlete. 6. den jsme v zarodečnem epitelu objevili take mnohojaderne buňky a jeho vakuolizaci. Po sedmi dnech s aplikaci MDOCTM jsme našli vyznamnou degeneraci zarodečneho epitelu zahrnujici mnoho znaků od vakuolizace epitelu po značne množstvi apoptotickych buněk. Take jsme nalezli velke množstvi infiltrovanych makrofagů. Nezazmamenali jsme žadne degenerativni znaky u kontralateralnich kontrol, ani u kontroly, kde jsme opět vratili varle do skrota (shame operated).

Abstract. We had studied morfological and histological changes of Wistar rat germinal epithelium after experimentally induced cryptorchidism. Our experiment had lasted seven days. Cryptorchic testes was injected by solution of MDOCTM. We used also tissues which was taken from other comparable studies but without MDOCTM for histological evaluation of shorter time intervals. Both size and weight was significantly reduced in cryptorchid testes compared to contralateral and control testes (22.4% and 52.6% respectively). We recorded degenerative changes of germinal epithelium including epithelial desintegration and release of immature and apoptotic cells, which was found in epydidimal tubules, even in one hour interval. Six days after operation, multinucleated cells and vacuolization of epithlelium also appeared. Seven days after operation (and aplication MDOCTM) we found a significant degeneration of germinal epithelium comprising features from vacuoles to apoptotic cells. A large number of macrophages also appeared in this interval. We didn't record any degenerative features in contralateral and control testes.